Purification and characterization of Hemolysin produced by local isolates of Vibrio cholera | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 10, Issue 1, February 2014, Pages 74-83 | ||
| Authors | ||
| Mohammed Ibrahem Nader; Amer Hani Razaq AL.Shammary; Muthanna Abdulkhader Al-Mahdawi | ||
| Abstract | ||
| The Hemolysin was Extracted from Vibrio cholera cultured on Brain Heart infusion by cold centrifugation 5000 r/min for 15 min at 4°C, and purified by several steps, including precipitation with 75% saturation ammonium sulphate, ion exchange chromatography using DEAE-cellulose and gel filtration on Sephadex G-100 column. The obtained purification fold and recovery were 24.26 unit /ml and 32.47% respectively. The molecular weight of the enzyme is about 19490 Dalton as determined by gel filtration. The optimum pH for activity and stability was 6 and 6-8 respectively. The enzyme retained its original activity when incubated at 20-45oC for 15 min, the activity of the enzyme decreased after enzyme incubation at 45oC. The maximum enzyme activity observed at 30oC | ||
| Keywords | ||
| Vibrio cholera; haemolysin purification; haemolysin characterization | ||
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