Mustafa Ali, R., N. Al- Thwani, A., H. Dhaer, F. (2014). Comparison between different methods for DNA Extraction for detection of Mycobacterium tuberculosis from sputum using Polymerase chain reaction. Alustath, 10(1), 51-59.
Ruqaya Mustafa Ali; Amina N. Al- Thwani; Fahim H. Dhaer. "Comparison between different methods for DNA Extraction for detection of Mycobacterium tuberculosis from sputum using Polymerase chain reaction". Alustath, 10, 1, 2014, 51-59.
Mustafa Ali, R., N. Al- Thwani, A., H. Dhaer, F. (2014). 'Comparison between different methods for DNA Extraction for detection of Mycobacterium tuberculosis from sputum using Polymerase chain reaction', Alustath, 10(1), pp. 51-59.
Mustafa Ali, R., N. Al- Thwani, A., H. Dhaer, F. Comparison between different methods for DNA Extraction for detection of Mycobacterium tuberculosis from sputum using Polymerase chain reaction. Alustath, 2014; 10(1): 51-59.

Comparison between different methods for DNA Extraction for detection of Mycobacterium tuberculosis from sputum using Polymerase chain reaction

Diyala Journal For Pure Science
Article 1, Volume 10, Issue 1, February 2014, Pages 51-59
Authors
Ruqaya Mustafa Ali; Amina N. Al- Thwani; Fahim H. Dhaer
Abstract
Deoxyribonucleic acid (DNA) extraction methods from Mycobacterium tuberculosis using sputum samples were evaluated including boiling, Soled phase absorption, Triton and N- cetyl-N,N,N-Trimethial ammonium bromide (CETAB). CTAB method showed 70 % detection sensitivity with strong band on agarose gel. Boiling and Triton methods to be unsatisfactory because they yielded either low quantity or poor quality of DNA or were unable to remove inhibitors of DNA amplification.
Keywords
PCR; DNA extraction; Mycobaterium tuberculosis
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