PRODUCTION AND PURIFICATION OF EXTRA CELLULAR LIPASE FROM PSEUDOMONAS CEPACIA AND STUDY THE PARTIALCHARACTERIZATION . | ||
| Diyala Agricultural Sciences Journal | ||
| Article 1, Volume 5, Issue 2, December 2013, Pages 436-450 | ||
| Authors | ||
| Alyaa M.Abdelhameed; Mohamed K. Kither; | ||
| Abstract | ||
| Twelve isolates of Pseudomonas were obtained from thirty five(34%) from different species soil ,plants and river s water in al Muqdadia fields .Ability of lipase production by these isolates was screened Pseudomonas P5 was highest lipase producer identified as Pseudomonas cepacia . Lipase production conditions were studied .the highest production of lipase was observed when mineral medium containing 5% linseed oil inculcated with bacterial cells e ,ph 7.5 and incubated at40 c in shaking incubator120 r.p.m for 18 hr. Lipase was purified from bacterial cells by two steps including precipitation with ammonium sulfate and Ion exchange chromatography by DEAE- Sephadex-A25, fold of purification was 35 Uml with 36.5% enzyme recovery . The effect of some metal ions on lipase activity was studied .The Ca+2 increased lipase activity to 150%. The effect of specific substances on enzyme activity was investigated ,The result showed that Tween80 raised lipase activity to 145%. | ||
| Keywords | ||
| Inhibitors Pseudomonas cepacia; Lipase enzyme; Characterization; Pseudomonas cepacia; inhibitors | ||
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