Compression of two genomic DNA extraction methods from Sheep blood | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 13, Issue 0, April 2013, Pages 128-133 | ||
| Abstract | ||
| The DNA is the major factor that responsible of transporting genetic code and biochemical reactions because of that the researcher show specific interest about the methods of extraction and purification of it to be used in different techniques like genetic engineering and gene therapy as well as improving animals and plant production, our study was about evaluation of two DNA extraction methods from sheep blood to know the best one of them. The first method was the modified organic method by using Sodium Perchlorat instead of organic solvent (Ethanol, chloroform), The other method was the enzymatic method by using proteinase K, The modified method is depend on utilization of the sodium perchlorat advantage that come from its cheap price and low storage and shipping requirements, The result show that the modified method give a good DNA yield and need relatively short time while the enzymatic method give an excellent DNA yield. | ||
| Keywords | ||
| DNA; genomic; Sheep | ||
|
Statistics Article View: 102 PDF Download: 46 |
||