Detection of Sperm Deformity by Kruger Strict Criteria using Pre- staining Slides Before and After Post Coital Test | ||
| Journal of University of Babylon | ||
| Article 1, Volume 20, Issue 4, October 2012, Pages 1152-1158 | ||
| Authors | ||
| Saad S. Al-Dujaily; Sondos K.Alatrakji; Safaa Alhasani | ||
| Abstract | ||
| Objective : There are many methods to examine the sperm morphology .One of the most important method with high accuracy is Kruger strict criteria .The objective of this study is to :1-simplified the method and lowering the time consumption for examination. 2- find out any changes may occurs on sperm morphology through post coital test. Design: Prospective study Settings: IVF Institute, Al-Nahrain University, Baghdad-Iraq. Materials and Methods: Seventy four unselected couples were involved at the present work who attend the Infertility Clinic of the IVF Institute. Semen fluid analysis(SFA) was performed for all men as recommended by WHO(1999).According to the results of SFA, the samples were divided into normal and abnormal semen . Azoospermic, sever oligospermic , sever asthenospermic samples were excluded from this investigation . Special pre-stained slides( Testsimplet® slides, Warymart-Germany) were used for examination of sperm deformity .One drop of liquefied semen was mounted on warmed pre-stained slides and covered by standard cover slip .The slides were left for 5 minutes at room temperature and examined as described by Kruger strict criteria. When the results of men samples showed normal sperm morphology, Post coital test were done for their spouses with normal cervical mucus score. The results of sperm morphology obtained from PCT were recorded by using the Kruger criteria too. Results: There was 28 out of 74 semen samples with normal sperm morphology. Fifty two samples revealed abnormal sperm morphology when examined by Kruger criteria and 36 samples were abnormal by using staining method alone . The percentage of morphologically normal sperm using Kruger criteria pre-coital test was 14.8% and following PCT the percentage was 13.4%.The statistical analysis showed a significant(P<0.05) differences between them. Conclusion: It is concluded from the present study that some results of normal sperm morphology were changed in the cervix to abnormal sperm form by using Kruger criteria, even the cervical mucus showed normal score. This may interpret some unexplained infertility cases. At the same time, the study noticed that the examination by the stained slides and using Kruger criteria was so simple , easy to perform and the time required to prepare the samples is short to start reading the results. | ||
| Keywords | ||
| Kruger strict criteria; testsimplet slides; male infertility; post; coital test | ||
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