Purification of Exotoxin A from Locally Isolate of | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 12, Issue 1, June 2013, Pages 68-76 | ||
| Authors | ||
| Maha F. Majeed; Subhi J. Hamza; Sara K. Nafea | ||
| Abstract | ||
| Twenty-five isolates of Pseudomonas aeruginosa were isolated from wounds, burns, inflammation of the urinary tract(UTI), sputum and ear infections. These isolates were found producing exotoxin A as indicated by ELISA test. Isolates from different infections (burn, wounds and UTI) were selected according to their productivity. An amount of 0.045, 0.064 and 0.040 mg/ml protein which represent and 23.31, 29.73 and 19.01 ng/ml exotoxin A were produced respectively. Extoxoxin A was partially purified using precipitation by 90% ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B was using to detection the molecular weight for the purified toxin was estimated as 65 KD. | ||
| Keywords | ||
| Exotoxin A; Pseudomonas aeruginosa; ELISA test | ||
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