Serological study on diagnosis of brucellosis in buffaloes in middle and south of Iraq | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 5, Issue 2, December 2012, Pages 104-110 | ||
| Authors | ||
| E. J. Abdul Al- Hussain; S. H. Thaer | ||
| Abstract | ||
| This study was carried out to diagnose brucellosis in buffaloes in seven provinces in middle and south of Iraq (Baghdad, Salah-Eldin, Wasit, Babil, Thiqar, Missan & Basra). 524 blood samples were obtained from 134 aborted buffaloes, 300 non aborted and 90 pregnant ones, 25 vaginal swabs, 9 aborted fetuses and 134 milk samples were also collected from aborted and lactating buffaloes for bacterial isolation and milk ring test performance respectively. Four serological tests were performed to detect brucella antibodies in serum and milk. These are Rose Bengal test (RBT), Tube agglutination test (TAT), complement fixation test (CFT), and Milk ring test (MRT). The results revealed that among vaginal swabs and aborted fetuses only two feti were suitable for bacterial isolation. Brucella abortus biotybe-3 was isolated from stomach contents of one of them. RBT revealed 10.4% of aborted cases, 7% of non aborted and 3.3% of pregnant, the over all percentage of infected cases was 7.2%. TAT revealed 8.9% of aborted cases, 4.3% of nonaborted and 1.1% of pregnants, the over all percent of infected cases was 4.9%. The agreement between RBT and TAT in detecting of infected cases was 68.4%. CFT revealed 12.6% of aborted cases, 7.6% of non aborted and 4.4% of pregnants, the over all percent of infected cases was 8.3%. The agreement between RBT and CFT in detecting of infected cases was 94.7%. MRT was performed on 134 milk samples, and revealed 11.9% of aborted cases. The agreement between MRT and RBT in detecting of infected cases was 92.8 %. In conclusion, RBT and MRT are rapid sensitive screening tests for diagnosis of brucellosis in buffaloes. Both tests with CFT are cardinal tests for diagnosis of brucellosis in small and large ruminants | ||
| Keywords | ||
| Brucellosis; buffaloes | ||
|
Statistics Article View: 208 PDF Download: 86 |
||