Diagnosis of Toxoplasmosis among couples by immune detection and genetic evaluation of Toxoplasma gondii | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 5, Issue 2, December 2012, Pages 143-148 | ||
| Authors | ||
| Sh. A. Mohammed; A. H. Abudulla; H. K. Buniya; M. M. Mkhlaf | ||
| Abstract | ||
| In this study, 27 married patients were totally examined for the presence of antibodies against Toxoplasma gondii by using a standard commercial enzyme-linking immunosorbent assay (ELISA) and Polymerase Chain Reactin (PCR) technique was used evaluate the genome of this parasite. Of 27 whole blood of married samples, nested PCR was positive in 22 (77.77%) by got the 96 bp DNA fragment, 9(40.9%) of them appeared with IgM and 6 (27.27%) of no anti-Toxoplasma, while 7 (31.81%) was positive nPCR results revealed both IgM+ and IgG+ antibodies, and 5 (22.22%) were negative results obtained by ELISA and nPCR | ||
| Keywords | ||
| Toxoplasma gondii; PCR; ELISA | ||
|
Statistics Article View: 59 PDF Download: 39 |
||