Comparison between total DNA concentration in the vaginal discharge in pregnant and non pregnant Awassi ewes | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 5, Issue 2, December 2012, Pages 54-56 | ||
| Author | ||
| S. A. Hatif | ||
| Abstract | ||
| The study was conducted on 20 Iraqi Awassi ewes (10 pregnant and 10 non pregnant), aged between (2-5) years old. Utilized Proteinase K buffer solution (100 mM NaCl, 1 mM EDTA, 10 mM Tris-HCl pH 8.0) for DNA extraction. The vaginal fluid were taken from the animals in order to estimate DNA concentration specterophotometrically. The results showed that the concentration of DNA was 4.2579 gm / l in pregnant ewes, while it was 4.3601 gm/ l in non pregnant ewes, it was concluded from this study there was no significant difference in DNA concentration in the vaginal fluid between pregnant and non pregnant Awassi ewes in p> 0.05. | ||
| Keywords | ||
| DNA extraction; vaginal discharge; DNA | ||
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