VEGF EXPRESSION IN TROPHOBLASTIC TISSUE OF WOMEN WITH SPONTANEOUS MISCARRIAGE AND INFECTED BY TOXOPLASMA GODNII USING INSITU HYBRIDIZATION | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 2, December 2012, Pages 494-502 | ||
| Author | ||
| Nidhal A. AL-Mohaimen | ||
| Abstract | ||
| A total of fifty women, aged between (16-42) years, were involved in this study. Enzyme linked immunosorbent assay (ELISA) test was used for the detection of specific IgM (using serum samples) and insitu hybridization method was used for the detection of VEGF m RNA in trophoplastic tissue. Samples were classified into three groups: Group A-patients with spontaneous miscarriage and Toxoplasma godnii positive (n= 20 women), with a mean age of(23.8±1.631);Group B-patients with spontaneous miscarriage and Toxoplasma godnii negative (n=20 women), with a mean age of (25.5± 1.60); Group C- control group, women with induced abortion for medical causes (n=10 women), with a mean age of (26.4± 1.628). The highest percentage of Vascular Endothelial Growth Factor mRNA expression (65.30± 3.587)% was within the induced abortion group, while the lowest expression was within (A) and (B) group (35.45± 2.184)% and (37.65± 2.964)% respectively. These results indicated that VEGF is essential factor in the process of blood vessel formation in the successful pregnancy, and its low level in miscarriage groups indicated its role in the etiology of miscarriage irrespective of toxo status. | ||
| Keywords | ||
| Trophoblastic tissue; Spontaneous miscarriage; Toxoplasma gondii; VEGF; in situ hybridization | ||
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