Distribution of aminoglycoside resistance mediated by16S rRNA Methylation between locally isolated ofEscherichia coli and Pseudomonas aeruginosa | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 23, Issue 5, July 2012, Pages 13-24 | ||
| Author | ||
| Israa Mohamed safi al- kadmy | ||
| Abstract | ||
| Clinical isolates (100) of (58) Escherichia coli and (42) Pseudomonas aeruginosa were collected from patients with different infections at Al-Kindey teaching hospital , Al-Kadhymia teaching hospital, Ibn-Albalady hospital and Al-Imam-Ali hospital in Baghdad. These isolates were diagnosed using different morphological and biochemical test followed by the complementary API 20E. Results of primary screening test for aminoglycoside resistance using disk diffusion method revealed that all the isolates conferring multidrug resistance and the highest resistance was against kanamycin, while the lowest was against amikacin .Detection of Extended spectrum β–lactamase( ESβLs) was also preformed and the results showed that 84% of the isolates gave positive result. Highly resistant isolates (20 for each ) were selected for the genetic study using polymerase chain reaction techniqe (PCR) to determine aminoglycoside resistance mediated by methylation 16S r RNA beside detection blaCTX –M gene responsible for ESBLs production .Two 16S rRNA methylase genes were amplified ,the armA (846 bp)and rmtD (500 bp) beside amplifying blaCTX –M gene ( 550bp) .Out of 20 E.coli isolates ,16(80%)gave positive results for armA gene, while non of P.aeruginosa harboured this gene .For rmtD gene, only one isolates of E. coli (5%) was positive, while 3 isolates of P.aeruginosa (15%) possess this gene . For blaCTX –M gene ,it was detected in all E.coli isolates (100%) and were detected in 17(85%) for P.aeruginosa. This is the first report in Iraq for the emergence of 16S rRNA methylases among E.coli and P.aeruginosa in correlation with ESβLs production | ||
|
Statistics Article View: 29 PDF Download: 8 |
||