Study the Inhibiting Effects of Bacillus subtilis which Isolated from Iraqi Soil on some Isolates of Staphylococcus aureus and Escherichia coli which Isolated from wounds and Burns | ||
| journal of the college of basic education | ||
| Article 1, Volume 18, Issue 75, September 2012, Pages 729-742 | ||
| Author | ||
| Shahrazad Najem Abdu-Allah Al-Shuwaili Department of Biology- College of Science - University of Al-Mustansiryia | ||
| Abstract | ||
| Form various soil samples which were collected from different areas of Iraq (Baghdad , Najaf , Karbala ,Babel) ,(50) Bacillus isolates were obtained , (8) Isolates belong to Bacillus subtilis. All isolates were subjects to the microscopical , cultural and biochemical examination which included ability to produce : Catalase, oxidase, growth in anaerobic condition, growth in the presence of (2%,5%,7%,10%) Nacl ,indole production test ,citrate utilization test ,voges-proskaur test ,starch hydrolysis ,glucose with gas and ability to ferment (7) carbohydrate sources. Capability of these isolates to produce inhibitory materials when grown in Nutrient broth against tow pathogenic bacteria isolated from burns and wounds (Staphylococcus aureus and Escherichia coli )were tested by used of the agar well diffusion method . To increase their activity, filtrates of the isolates that grown in liquid medium were concentrated .Four isolates were chosen due to their highest antibacterial activity .The results of this study showed that: unconcentrated filtrates of bacterial isolates did not show any inhibitory effect against pathogenic bacteria in the study , except (3) filtrates belonged to Bacillus subtilis-1, Bacillus subtilis-2 and Bacillus subtilis-4 which gave low inhibitory activity against Staphylococcus aureus .while the concentration of all bacterial filtrates for one and two-fold exhibited their activity .The concentration of all bacterial filtrates showed high inhibitory effect against Staphylococcus aureus while showed low inhibitory effect against the isolates of Escherichia coli | ||
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