USING ELISA TECHNIQUE IN DETECTION OF MOLD CONTAMINATION OF TOMATO PAST | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 2, December 2012, Pages 163-174 | ||
| Author | ||
| Abdul Ghani I. Yahya | ||
| Abstract | ||
| Indirect ELISA was used for determining antibodies titer in antisera using Anti – IgG – HRP (Goat Anti – IgG-Horse Radish proxidase), the titer of these anti bodies for Aspergillus flavus, Rhizopus stolonifer were 1/160, 1/80. Conjugate was prepared by labeled antisera against A. flavus , R. stolonifer (After precipitation by ammonium sulfate) with alkaline phosphatase, titration of conjugate to each one was carried out using ELISA test, results showed that the titer of A. flavus conjugate was 1:10 and R. stolonifer conjugate was 1:5 The sandwich ELISA test was used for detecting several concentrations of A. flavus, R. stolonifer and their extracts in carbonate buffer solution pH 9.5 involved: 25, 12.5, 6.25, 3.125, 1.56, 0.78, 0.39 micrograms per milliliter. ELISA test also used in detecting their concentration: 1, 2, 3 micrograms of these two fungi per milliliter of tomato paste diluted 1:5 diluted with PSB solution results showed that the absorbance of these concentration in carbonate buffer was more than in tomato paste and positive correlation was found between concentration of mold and the absorbance , ELISA test was compared with biological method (plating culture) for detecting the three concentrations of mold in tomato paste, results showed that ELISA test was more sensitive than plating culture which detect viable mold spores only. | ||
| Keywords | ||
| Fungal contamination; ELISA; Tomato paste | ||
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