DETERMINING THE CELLULASE GENE LOCATION OF SOME PSEUDOMONAS SPECIES ISOLATED FROM SOIL AND STUDYING THE POSSIBILITY TO INCREASE CELLULASE DEGRADATION BY PLASMID DNA AMPLIFICATION TECHNIQUE | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 2, December 2012, Pages 151-162 | ||
| Author | ||
| Rayan M. Faisal | ||
| Abstract | ||
| Twenty six cellulose-degrading bacterial isolates were isolated from different soil samples from Nineveh governorate, these isolates were checked for antibiotic resistance towards Tetracycline, Nalidixic acid, Cefalexin, Ampicillin and Chloramphenicol, according to their resistance pattern eight of them were chosen for diagnosis. The study found that all these isolates belonged to Pseudomonas putida. Curing by elevated temperature was carried out to identify the cellulose degradation gene location, curing percentage of some isolates were between (4-31%) which may give a fact that these isolates may carry cellulose genes on their plasmids. On the other hand some isolates failed to be cured probably because their cellulose genes were chromosomal. Plasmid amplification by 150µg/ml chloramphenicol was studied for all isolates that carried plasmid for cellulose degradation, we found that the isolate (19) had the ability to amplify its plasmid content. Certification that cellulase plasmids were amplified was done by comparing the CO2 evolution for the original and the amplified isolate per week. | ||
| Keywords | ||
| Cellulase gene; Location; Pseudomonas | ||
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