Study the Bacteriocin Production from Enterococcus Faecium Isolated From Clinical Sources | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 23, Issue 3, April 2012, Pages 1-10 | ||
| Authors | ||
| A. Jasim; | ||
| Abstract | ||
| Two hundred ninety clinical samples were collected from patients of Baghdad hospitals,distributed into(177) urine and (113) stool samples.Bacteriological and biochemical detection tests showed that only 27 isolates (9.31%) were identified as Enterococcus faecium.Antimicrobial susceptibility results showed a high resistance to Nalidixic acid (81%) and Tetracycline (63%) ,while (48%) of the isolates were resistant to Ciprofloxacin. E.faecium showed low resistance to Trimethoprime (19%) and (7%) to Gentamycin. All isolates produce bacteriocin (100%) with a wide range effect on gram positive and negative bacterial growth,with diameter of inhibition zones (8-15)mm and (9-27)mm on the same isolates of species. E.faecium EH1 chosen as the best producer and solid and broth Man Ragusa sharp medium was the best medium for production at )16-45(Cº.Crude bacteriocin was stable at temperature(80,100,121Cº) for (15 ,30) minutes and in pH range (2-8) and storage in temperature (0,4Cº,37) for week.Partial purification with ammonium sulphate in percentage 40% was the best with a specific activity (44.137)unit /mg protein and protein concentration (290)mg/ml.The partial purified bacteriocin was stable at 80Cºfor 30 minutes and 100Cº for( 5,15) minutes.Its activity was stable in pH (5.5-8.5) and storage at 0Cº for week. | ||
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