Effect of Water & Methanol Extracts of Turkish Propolis Against Some Species of Pathogenic Bacteria | ||
| IRAQI JOURNALOF COMMUNITY MEDICINE | ||
| Article 1, Volume 25, Issue 3, September 2012, Pages 210-215 | ||
| Authors | ||
| Alyaa M. Abdul Hadi; Naksheen M. Mahmood | ||
| Abstract | ||
| Abstract Background: Propolis (bee glue) is a mixture of wax and resin. This products have some properties such as bactericidal and fungicidal and also it was could be used as an alternative treatment against infections. Objective: The study was shed light to evaluate the effect of alcoholic and water (hot& cold) propolis extract against some pathogenic bacteria and also to investigate the antimicrobial activity "in vitro". Material & Methods: Both alcoholic and water (hot & cold) extract from Propolis which used to obtained the inhibited growth and the effectiveness against some bacterial strain ( Escherichia coli, Pseudomonas aeruginosa, Proteus Spp., Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilus ) and this effected will be measured by the minimum inhibitory concentration (MIC) on the bacterial species and this occurs by using agar distribution methods with Propolis extract in serial concentration by treat each of bacteria with different concentration (6.2,12.5,25,50,75,100,125,150and 200mg/ml). Results: The result showed that the alcoholic extract which have more effective as an inhibitor on the growth compared with water extract. When used alcoholic Propolis extract the results obtained that the diameter of inhibition zone was(6 ,18 .and 19 mm) respectively when treat each of bacteria (Escherichia coli,., Staphylococcus aureus, Streptococcus pyogenes,) with different concentration (100,125, 150, 200)µg/ml .While in the type of bacteria (Proteus Spp) the inhibition zone was 6mm in diameter when used the concentration 125 µg/ml from extract Also the results obtained there was a positive effect against Pseudomonas aeruginosa, and Bacillus subtilus when treat it with propolis extract in the concentration (6.2, 12.5, 25,50)µg/ml and the inhibition zone showed (8and5mm) in diameter respectively, While the inhibition zone for Klebsiella pneumonia was (6mm) in diameter when treat it with the concentration (6.2) µg/ml Conclusion: The results in this study refer to the ability of propolis extract to inhibitory effect on the growth and effectiveness of the bacteria and the effect depend used... | ||
| Keywords | ||
| Turkish propolis; methanol extract; antimicrobial activity | ||
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