Effect of In-vitroSperm Activation Techniques and Albumin Concentrations on Human Sperm Parameters and Chromatin Structure | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 5, Issue 2, December 2012, Pages 161-167 | ||
| Authors | ||
| Abd Al- Ameer N.Ghaloub; Muhammed- Baqir M.R.Fakhrildin; Nadia N. Nasser | ||
| Abstract | ||
| Current study used the acridine orange test to investigate the effect of two techniques of human sperm preparation involved direct swim up technique and centrifugation swim up technique on the integrity of sperm chromatin structure, and also the supplementation of two concentrations of Human serum albumin (HSA) in the culture medium that has been used in the previously mentioned methods. Assay results demonstrated that, both techniques reduce the percentages of isolated or activated spermatozoa with fragmented DNA as compared with their percentages pre-preparation, whither this decrement was significant or not (P=0.05). Also we found that using culture medium supplemented with concentrations of HSAenhance the isolation of spermatozoa with intact DNA, and (10%)HSA was the better.Therefore, the present study concluded that neither direct swim up nor centrifugation swim up techniques with single centrifuge step (559g) for 8minutes can cause further DNA damage in the activated spermatozoa by both, also the involvement of HSA in sperm preparation media enhances sperm parameters and the isolation of spermatozoa with intact DNA in-vitro. | ||
| Keywords | ||
| DNA fragmentation; sperm activation; swim up | ||
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