PREVALENCE AND ANTIMICRIBIAL SUSCEPTIBILITY OF ESCHERICHIA COLI O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCES IN BASRAH PROVINCE | ||
| Basrah Journal of Veterinary Research | ||
| Article 1, Volume 11, Issue 2, December 2012, Pages 47-55 PDF (0 K) | ||
| DOI: 10.33762/bvetr.2012.68915 | ||
| Authors | ||
| Bassam Y. Khudaier; Basil A. Abbas; Khulood A. Khleel | ||
| Abstract | ||
| The present study assessed the prevalence of Escherichia coli O157 in diarrhea patients ,beef, and raw milk. A total of 675 samples were inoculated in trypticase soy broth to enhance the growth of E. coli O157:H7. Out of total samples 73.5% isolated as E. coli then cultured on Sorbitol MacConkey agar ,31.8% non fermenting sorbitol (NSF) E. coli colonies were isolated and confirmed by specific biochemical tests . From NSFEC 13.7% were diagnosed as E.coli O157:H7 by serological test ,the result revealed no significant differences in the level of contamination with E. coli O157:H7 between beef ,stool and milk .The isolated bacteria were tested for antibiotic susceptibility test which showed resistance 100% to cephalothin ,cefoxitin , cefixime, trimethoprim , amoxicillin, azithromycin, and amoxicillin/clavulanic acid and sensitive 100% to ciprofloxacin ,imepenim ,nitrofurantion gentamycin and amikacin . No major differences in antibiotic susceptibility patterns among the isolates were observed. | ||
| Keywords | ||
| Escherichia coli O; Sorbitol; Milk | ||
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