U.Mohammd, T., M.Al-Mhana, N., S. Saleh, S., F.Abd Al-Zhra, A. (2012). Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes. Alustath, 16(4), 453-461.
Tagreed U.Mohammd; Nadia M.Al-Mhana; Saad S. Saleh; Azhaar F.Abd Al-Zhra. "Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes". Alustath, 16, 4, 2012, 453-461.
U.Mohammd, T., M.Al-Mhana, N., S. Saleh, S., F.Abd Al-Zhra, A. (2012). 'Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes', Alustath, 16(4), pp. 453-461.
U.Mohammd, T., M.Al-Mhana, N., S. Saleh, S., F.Abd Al-Zhra, A. Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes. Alustath, 2012; 16(4): 453-461.

Comparison Study of (urinary & serum) AST Activity from Patients with type 2 diabetes

Journal of Engineering and Sustainable Development
Article 1, Volume 16, Issue 4, December 2012, Pages 453-461
Authors
Tagreed U.Mohammd; Nadia M.Al-Mhana; Saad S. Saleh; Azhaar F.Abd Al-Zhra
Abstract
Aspartate aminotransferase was purified from urine and serum of patients with type 2 diabetes in a 2 steps procedure involving dialysis bag and sephadex G-25 gel filtration (column chromatography). The enzyme was purified 346.23 fold with 1467% yield and 3.46 fold with 142.85% yield in urine and serum of patients with type 2 diabetes respectively. The purified enzyme showed single peak. The results of this study revealed that AST activity of type 2 diabetes urine and serum increased significantly (p<0.001) compared with control group.
Keywords
Aspartate aminotransferase; Purification; Diabetes
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