Purification , characterization of chitinase produced from Bacillus subtilis and inhibitory activity on some fungi | ||
| journal of kerbala university | ||
| Article 1, Volume 8, Issue 2, June 2012, Pages 166-173 PDF (0 K) | ||
| Abstract | ||
| Bacillus subtilis with highest production of chitinase was selected among other Bacillus sp. Chitinase was extracted from culture media contain shrimp and fish-shell powder as the major carbon. Enzyme was purified by anion exchanger (DEAE) and Sephadex G-50 Gel filtration with yield and Purification fold, 33.18% and 11.01 respectively. Optimum PH for activity and stability was 6,5 and 5.5-8 respectively, The purified enzyme had Mw. 37 Kd. Inhibitory activity was 100% against some molds after incubation for 2h with sterilized chitinase solution (Unit). | ||
| Keywords | ||
| Bacillus subtilis; chitinase; Purification; fungal lysis | ||
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