Identification of Enterococcal Species by Polymerase Chain Reaction Technique, with Study of Some Immunological Features | ||
| Medical Journal of Babylon | ||
| Article 1, Volume 9, Issue 4, December 2012, Pages 824-832 | ||
| Author | ||
| Zainab A. Chabuck Alaa H. Al-Charrakh | ||
| Abstract | ||
| Objectives: Detection of genes responsible for identification of enterococci to the species level using PCR, determination of enterococcal serum resistance and phagocytic index. Materials and methods: seven enterococcal isolates were obtained from two hundred urine samples. After identification of enterococcal isolates by biochemical tests, all of them were subjected to PCR technique using species-specific PCR primers for E. faecalis and E. faecium, enterococcal serum resistance and phagocytic index were detected. Result: This study revealed that five isolates (71.43%) reacted with ddlE. faecalis; whereas the remaining (28.57%) isolates reacted with ddlE. faecium. Also, revealed that phagocytic index of two E. faecalis and one E. faecium isolates were 27%, 25% and 19%, respectively, while one E. faecium isolate showed phagocytic index as 8%. Moreover, enterococcal serum resistance showed that E. faecalis was more sensitive to the serum as compared to E. faecium. Conclusion: Enterococcal isolates can't be easily identified to the species level by conventional or traditional methods but it can be easily identified by species-specific PCR primers ddlE. faecalis and ddlE. faecium. Enterococcous faecalis tends to be more sensitive to innate immune parameters as compared to E. faecium. | ||
| Keywords | ||
| faecalis and E; faecium species; specific PCR primers; Enterococcous spp; Polymerase chain reaction | ||
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