Radiation Stimulates Nitric Oxide Release from Isolated Protein of Patients with End Stage Renal Failure: In Vitro Study | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 23, Issue 2, March 2012, Pages 1-10 | ||
| Author | ||
| Marwan S. M. AL-Nimer | ||
| Abstract | ||
| It is well known that exposure of human protein to the ultraviolet radiation resulted in denaturation. Few data explore the importance of the production of non enzymatic nitric oxide from endogenous sources. This study is aimed to explore the effect of ultraviolet radiation, monochromatic light and day light on the production of non-enzymatic nitric oxide from isolated serum protein of patients with end stage renal failure. The sera protein of 12 patients with end stage renal failure were isolated by precipitation methods and exposed to UVA (365 nm), UVC (254 nm), monochromatic light (sodium lamp; 589.3 nm) or sun light for 2hours. Nitrogen species in term of nitric oxide and peroxynitrite were determined in patients sera, and in the protein aliquots before and after irradiation. Production of non-enzymatic nitric oxide was significantly observed in protein aliquot exposed to UVA (1586±763.8 μmol), monochromatic light (1047.7±397.7 μmol) and UVC (919.3±794.6) compared to that exposed to the sun light (687.7±531.4 μmol). It concludes that ultraviolet radiation or monochromatic lighting are good stimulator of nitric oxide production from isolated sera protein of patients with end-stage renal failure. Such effect may produce harmful as well as beneficial effect. Key words: Nitric oxide. Serum protein, Renal failure | ||
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