Emergence And Detection Of Ampc Gene By Polymerase Chain Reaction From Sepsis Serratia Marcescens | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 23, Issue 1, January 2012, Pages 39-54 | ||
| Authors | ||
| ; im R. Ali | ||
| Abstract | ||
| Two hundred blood sample collected from neonates with clinical sepsis were enrolled in the study between March and November 2010 from welfare pediatric hospital and Baghdad hospital neonatal intensive care unit. Twelve (12.24%) cases of Serratia spp. bacteremia occurred, 11(11.22%) of them belong to S. marcescens . This study detected high isolation value of S. marcescens and this result may be point to epidemic outbreak with these bacteria in intensive care units. Of the 11 S. marcescens isolates examined, only 3 (27.28%) produced prodigiosin pigment .This work describes a low frequency of isolation of pigmented S. marcescens from clinical specimens, indicating that non-pigmented strains are clinically more significant. The results showed that all S. marcescens strains were resistant (100%) to Ampicillin, Amoxicillin+ Clavulanic acid , Cloxacillin, Cefoxitine and Ceftazidium , but all sensitive to Imipenem and Azithromycin and varied in their response to other antibiotics used in this study. This study showed there is significant difference between prodigiosin production and antibiotic resistance pattern; the low resistances of the isolates were concentrated in pigment-producing isolates. As per the classification mentioned ,out of 9 AmpC βL-producing isolates, flattening of zone was observed in 77.77% (7/9) isolates, indicating weak AmpC βL producers and indentation indicating strong AmpC βL producers in only 22.22% (2/9) isolates. Around 44.44% (4/9) of AmpC βL-producing strains were found to be S to Cefepime. Sensitivity among Class A isolates was 0 %( 0 /11), Class A + Class C 18.18% (2 /11), Class C (2/11) 81.81%. The result showed possible AmpC producers resistant to cefepime , these modifying in resistant pattern among classC beta-lactamase producer newly noted in Iraqi S.marcescens isolates..From 11 isolates 9 of them were positive screening for ampC by phenol type detection. While resistance gene detected in 2(18.18%) of these isolates. The result showed antibiotic phenotypes cannot accurately distinguish the resistance mechanisms caused by ampC or ESBL, and especially in ESBL-ampC combinations. However, PCR is a useful technique for the identification of the different types of resistance genes. | ||
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