IN VITRO PLANT REGENRATION OF STRAWBERRY | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 1, Volume 25, Issue 2, December 2012, Pages 1-16 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2012.66347 | ||
| Authors | ||
| Z. A. Saaid; M.A. Ibrahim; H. A. Al-Taha | ||
| Abstract | ||
| The present research was conducted at the Plant Tissue Culture Lab. - Agriculture College – Basrah University for the period from 1/12/2010 to 1/10/2011 in order to propagate strawberry plant (Fragaria ananassa) cv. Albion using leaf segment and petiole in vitro culture by direct and indirect organogenesis. The results of showed that leaf segment produced granular callus after two months of culture on MS medium supplemented with different concentrations (0.5, 1.0, 1.5, 2.0 and 2.5) mg.l-1 benzyl adenine with a fixed concentration of naphthalene acetic acid (0.2 mg.l-1). The MS medium supplemented with 1.5 mg.l-1 benzyl adenine led to the shoot formation. The results also indicated that petioles cultured with the same components of MS medium led to the formation of coherent and active callus increased the quantity of increasing the concentration of benzyl adenine. When it culture led to shoot production with the emergence of the roots of white reddish short down this shoots at a concentration of 2.0 mg.l-1 benzyl adenine with the emergence of adventitious shoots directly from cut zone of leaf base of petiole . The results also showed that the cultivation of petiole with leaf base in MS medium with the same components and concentrations with the replacement of the kinetin instead of benzyl adenine led to the formation of lobular callus at concentrations of 1.5, 2.0 and 2.5 mg.l-1 Kinetin that gave shoots when re-cultured, while the petioles did not continue to growth at 0.5 and 1.0 mg.l-1. kinetin. The results showed a lobular callus production on leaf segments when growing in the MS medium supplemented with at 1.0 and 2.0 mg.l-1 concentrations of benzyl adenine with a fixed concentration at 0.1 mg.l-1 of 2,4-D. When the induced callus re-cultured at 2.0 mg.l-1 benzyl adenine led to the shoots formation. | ||
| Keywords | ||
| Callus; Organogenesis; benzyl adenine; Kinetin; Petiole | ||
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