Detection of bovine parainfluenza type 3 virus in Iraq | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 3, Volume 36, Issue 0, March 2012, Pages 16-24 PDF (0 K) | ||
| Authors | ||
| Aida Bara Allawe; Anton Sabri Al-bana | ||
| Abstract | ||
| This study is considered the first study in our country to isolate bovine parainfluenza type 3 virus (BPIV-3) from naturally infected calves. BPIV-3 virus was isolated from nasal swabs collected from calves with respiratory disease. The virus was isolated and titrated in secondary embryonic bovine kidney (SEBK) cell culture; other cell cultures (secondary sheep testis and chicken embryos fibroblast cell cultures) were used for propagation of isolated virus. Examination of infected cell culture revealed giant cell formation and intracytoplasmic inclusions. The isolated virus agglutinated guinea pig’s erythrocytes and produces clear heamadsorption of guinea pig’s erythrocyte in infected SEBK cell culture. Virus titer was 2×102TCID50/0.1ml in the sixth passage and became 2×104.5TCID 50/0.1ml in the ninth passage. The new isolated virus was identified as BPIV-3 by heamagglutination inhibition test, serum neutralization test and indirect immunoflurescent technique, by using specific monoclonal and polyclonal antibody. The virus was sensitive to ether. High titer of BPIV-3 antibodies was detected in sera of infected animals during convalescent period by ELISA test. | ||
|
Statistics Article View: 52 PDF Download: 24 |
||