Detection of Human Cytomegalovirus (HCMV) among Infertile Male in AL-Najaf governorate | ||
| kufa Journal for Nursing sciences | ||
| Article 1, Volume 2, Issue 3, December 2012, Pages 123-128 | ||
| Authors | ||
| Saif Jabbar Yasir; Kareem Thamir Mashkoor; Ghanim Aboud Al-Mola | ||
| Abstract | ||
| The current study aimed to detect human cytomegalovirus among infertile individual. Blood and seminal fluid collected from 300 infertile male randomly , ELISA technique was used to know the activity of humoral immunity among them through measuring anti-HCMV IgM and IgG antibodies , the result revealed that 31 (11%) were positive for IgM result and 234 (78%) were positive for IgG. The PCR technique showed that out of 300 samples only 22 (7.4%) gave positive results, In case of control group, results were negative for anti- HCMV IgM antibodies in ELISA test and PCR technique. While IgG gave a weak positive results. Aim of the study: The present study was to study the relationship between the virus and infertility through detection of human cytomegalovirus (HCMV) infection in infertile male in AL-Najaf governorate by screening of anti-human cytomegalovirus IgM and IgG in the serum and detection of human cytomegalovirus DNA in seminal fluid, using PCR technique. | ||
| Keywords | ||
| HCMV; Infertile males; ELISA; PCR | ||
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