Trichoderma Isolates Ability to Produce Cellulase Enzyme and it's Role as Resistance Inducer to FungusMacrophomina phaseolina | ||
| Rafidain Journal of Science | ||
| Article 5, Volume 22, Issue 6, September 2011, Pages 46-59 PDF (0 K) | ||
| DOI: 10.33899/rjs.2011.6522 | ||
| Author | ||
| Nihal Y. Al-Murad | ||
| Abstract | ||
| The result of this study indicated that Trichoderma spp. culture filtrates have antagonistic activity against Macrophomina phaseolina where the isolate Tv1 gave the higher antagonistic activity with 66%. in addition to that the same isolates showed higher antibiosis activity with an average of 1 according to Bell scale, with reduced pH level in culture media while the isolate Thk20 recorded the higher biomass amount of 1.17 g/100 mL with sporeulation ability 7,58 /cm2. Trichoderma isolates showed significant differences in it's capacity to produce cellulase enzyme where the isolate TV1 produced the higher amount of enzyme 1,26 unit/g, followed by the isolates Tv2, Th1, Thk20 with 0.88, 0.28 , 0.28 unit/gm respectively. The result also showed that the ability of Trichoderma isolates to induce resistance in cowpea plants by raising in peroxidase activity to 1.52 unit /g f.w in Tv2 and in polyphenol oxidase activity with 3.03 in the same isolate treatment in addition to the pathogen with the isolate Thk20 recorded the higher amount of total phenols in plant 1.51 mg/g.f.w and 1.46 mg/g.f.w in the same isolate treatment in presence of the pathogen | ||
| Keywords | ||
| Trichoderma; Cellulase; peroxidase; Poly phenol oxidase; Induced resistance | ||
|
Statistics Article View: 285 PDF Download: 606 |
||