Effect of Different Treatments on Regeneration, Growth and Acclimatization of Ananas Plantlets Produced By Plant Tissue Culture 2-Application Effect of Culture Media and Urea Fertilizer on Growth and Acclimatization of In vitro Propegated Pineapple(Ananas Comosus.L.Meer.cv.Del Mont Plantlet. | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 13, Issue 1, July 2013, Pages 230-236 | ||
| Author | ||
| Abstract | ||
| This experiment was conducted at laboratory of plant tissues- Department of Horticulture and landscape designe –College of Agriculture- Basra University, during 2012- season to investigate the enhancement of the growth of in vitro propagated pineapple plantlets after acclimatization (to reach their proper size when they were transported under green house conditions) by studying the effect of various culture media and application of different levels of urea to find out the best media and proper concentration of urea for gowth of in vitro propagated pineapple (Ananas cmousus .L.Merr),cv. Del Mont plantlets . Results of the study revealed that plantlets grown in culture medium containing only peatmoss was significantly increased in plant length , number of leaves /plant, fresh weights of vegetative growth and roots which were 18,77cm,9.33/plant,1.68 and 0.44gm respectively as compared with other pineapple plantlets grown in culture medium containing only sand or medium containing sand: peatmoss (1:1) . Plants gown in medium containing peatmoss had dark green color as compared with other plants gown in other media. The viability of plants grown in peatmoss media was 100% whereas plants grown in sand: peatmoss had 75% and 50% for those grown in media containing only sand. Fertilizer treatment of urea 2% resulted in significant increase in length of plants , number of leaves,vegetative growth and roots fresh weight, length and number of roots which were 14.83 cm,14.33/plant, 2.66gm,0.17gm,7.00cm,and 9.33/plant respectively ,as compared with other treatments (%0,%0.5,%1,%1.5) urea.The lower leaves that nearest to soil surface became brownish color and dried as the concentration of urea was increasing in soil of pots. | ||
| Keywords | ||
| Acclimatization; pineapple; urea; culture media | ||
|
Statistics Article View: 36 PDF Download: 11 |
||