Constructing and Building –up a home Made Semi-automated Ion Chromatography | ||
| basrah journal of science | ||
| Article 1, Volume 30, Issue 1, January 2012, Pages 56-65 PDF (0 K) | ||
| Author | ||
| K.H .Al-Sowdani and H.N.K. Al-Salman | ||
| Abstract | ||
| Ion Chromatography (IC) is a chromatographic separation and measurement of ionic species. This technique combines an ion – exchange chromatographic separation with simultaneous conductometric detection for the determination of anions, cations and other ionic species. Single – column IC system equipped with a conductivity detector and with a home-made flow cell 12.5 µl sample volume was constructed and build-up from simple and inexpensive parts readily available in our analytical laborites .The approach of single-column IC instrumentation depend on the small differences in the conductivity between sample ions and the prevailing eluent .To amplify these difference , low capacity exchanger are used for the home-made separation column ( 3 mm ID and 300 mm length ) which permit elution with low electrolyte concentration . The home-made single column IC has been applied to separate and determination of 25 µl of injecting, anions ( F- , Cl- , Br- , NO2- , NO3- , SO4-2 and PO4-2 ) and cations ( Li+ , Na+ , K+ , NH4+ ) . The accuracy of the method was examined by performing a recovery experiments using standard additions method. The results were compared and good agreement was obtained (94.28- 100.58) %. | ||
| Keywords | ||
| Home; made ion chromatograph; single column; Anions; Cations | ||
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