Effect of Some Chemical and Biological Treatments on The Growth of Aspergillus niger Van Tieghem Causing Black Mould Disease on Onion | ||
| journal of kerbala university | ||
| Article 1, Volume 8, Issue 0, March 2012, Pages 185-196 PDF (0 K) | ||
| Abstract | ||
| This study aimed to find the effective means to protect onion bulbs from Black Mould disease caused by the fungus Aspergillus niger in natural circumstances storage through testing the efficiency of some chemical treatments (Moncut, Urea) and biological treatments (Trichoderma harzianum, T. viride)) on the growth of A. niger. The results showed the efficiency of chemical and biological treatments that used in the inhibition of radial growth of A. niger on the Potato Dextrose Agar (PDA) as the percentage of inhibition reached 100 % at concentration 2 gm/L for Moncut, As for Urea, the rate of the diameters of the colonies at the concentration 15 gm/L reached about 13.97 mm with percentage of inhibition reached 84.47 %, while the rates of the diameters of the colonies reached 12.16 mm with percentage of inhibition reached 86.48 % at concentrations 15 % in the treatment of antagonistic fungus filtrate T. harzianum and reached 13.43 mm with percentage of inhibition reached 85.07 % at concentration 15 % in the treatment of antagonistic fungus filtrate T. viride in measuring with the Control treatment which the rate of the diameters of the colonies reached 90.00 mm, The results also showed that T. harzianum and T. viride have a higher antagonism ability against A. niger on (PDA). The results of the storage experiment had cleared efficiency of chemical and biological treatments that used in reducing the percentage of bulbs infection with fungus A. niger after the expiration of the storage which reached 3 months, and was treated bulbs with Moncut are more efficient which reduced the percentage of bulbs infection to 4.34 %, followed by treatment antagonistic fungus filtrate T. harzianum, which reduced the percentage to 12.76 %, and the results also showed that no significant differences between the antagonistic fungus filtrate T. viride and Urea treatments in reducing the percentage of bulbs infection at the level of possibility 5 % as reached (19.14-22.91) % respectively in measuring with the treatment of the Control which the percentage of bulbs infection reached 62.5 %, As for the effect of chemical and biological treatments on the weight of the bulbs, the results showed the efficiency of various treatments in maintaining the normal rate of bulbs weight as reached 493.6 gm in the treatment of Moncut while ranged between (478.5-485.8) gm in the treatment of antagonistic fungi filtrate T. viride and T. harzianum respectively, while reached 435.4 gm in the treatment of Urea in measuring with treatment of the Control which was the percentage of bulbs infection reached 284.2 gm. | ||
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