The Relevance of Glycosylated Hemoglobin with Oxidative Stress in Insulin Resistant Type 2 Diabetes Mellitus | ||
| Kerbala Journal of Medicine | ||
| Article 21, Volume 5, Issue 11, June 2012, Pages 1334-1342 PDF (0 K) | ||
| Authors | ||
| Majid Kadhum Hussain; Dhafera Jaffer Abed-Ali; Dhafera Jaffer Abed-Ali | ||
| Abstract | ||
| The present study was conducted to evaluate the relevance of glycosylated hemoglobin with oxidative stress in insulin resistant type 2 diabetes mellitus. To achieve this aim, 93 type 2 diabetic patients of ages 38-84 years were recruited. In addition, 19 apparently healthy individuals with ages 30-60 years, were enrolled as a control group.The concentration of fasting blood glucose (FBG), triglyceride (TG), insulin, glycosylated hemoglobin (HbA1c), malodialdehyde (MDA) and glutathione-S-transferase (GST) were measured in sera of patient and the control groups. Fasting blood glucose and triglyceride levels were estimated by spectrophotometeric methods using enzymatic procedures. Insulin level was estimated by enzyme linked immunosorbant assay (ELISA) method. HbA1c level was determined by an ion exchange chromatographic method, while MDA and GST levels were measured by spectrophotometeric procedures. Insulin resistance was evaluated by four methods. They include homeostatic model assessment (HOMA), quantitative insulin check index (QUIKI), McAulye (McA), and fastinge insulin (FI) methods. Insulin resistance was found in 79 (84.9%), 63 (73.4%), 52 (55.9%) and 35 (37%) out of the 93 diabetic patients by HOMA, QUICKi, McA and FI methods respectively. Type 2 insulin resistant (79) diabetic patients (IRP) that obtained through the HOMA method were assessed for the HbA1c, MDA and GST levels in comparison to the insulin sensitive patients (ISP) as well as to the control group. The analysis of the data revealed a significant increase (p<0.001) of HbA1c levels in the IRP group when compared with those of The Relevance of Glycosylated Hemoglobin….. Majid Kadhum Hussain kerbala J. Med. Vol.5, No.1, Dec, 2012 Page the control group, while the insulin sensitive group (ISP) couldn’t show significant variation when compared with those of the IRP. The data of MDA failed to give significant variation. However, a significant elevation of GST concentration were observed in the IRP group with respect to those of the control group (p<0.005). On the other hand significant variations could not be obtained among the ISP and IRP. These data suggested that oxidative stress changes are independent on insulin resistance in type 2 diabetes mellitus | ||
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