MOLECULAR DIAGNOSIS OF KLEBSIELLA PNEUMONIAE K1 AND K2 SEROTYPES ISOLATED FROM IRAQI CHILDREN INFECTED WITH SEPTICEMIA | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 1, June 2012, Pages 14-23 | ||
| Author | ||
| Yasameen A. Kareem Norrya A. Ali | ||
| Abstract | ||
| ABSTRACT Different bacteria were isolated and diagnosed from 200 samples infected with septicemia, taken from children attended IBN–ALBALADY hospital. For bacterial diagnosis the Bact/ALERT 3D and VITEK 2 were used. PCR with specific primers was used to detect the serotypes K1 and K2 of K. pneumoniae. Different bacterial genera in addition to K. pneumoniae were isolated such as Staphylococcus aureus, Enterococcus faecium, Salmonella typhi, Enterobacter cloacae, Acinetobacter lwoffii, E. coli. It was found that Staphylococcus aureus was the more dominant among gram positive bacteria (15 isolates) while K. pneumoniae showed the highest isolated number among the gram negative bacteria (17 isolates). Results of K. pneumoniae identification using PCR technique showed that of 17 K. pneumoniae isolates two isolates belong to K1 serotype, and two isolates belong to K2 serotype which gave DNA band of 1282 bp and 535 bp in size representing magA gene and rmpA gene respectively | ||
| Keywords | ||
| magA; rmpA; Serotypes | ||
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