IDENTIFICATION OF TRICHOMONAS VAGINALIS USING MOLECULAR METHODS IN IRAQI INFECTED WOMEN | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 11, Issue 1, June 2012, Pages 51-64 | ||
| Abstract | ||
| ABSTRACT There are different methods for identification and detection of Ttrichomonas vaginalis which causes trichomonasis. In this study Polymerase chain reaction (PCR) for amplification of 18S rRNA gene was used. Three hundred and four vaginal swabs were collected during the period (December 2010- September 2011) from hospitals of north east of Baghdad city from ladies complained of genital tract infections. There were 148 cases suspected to be trichomonasis, among these suspected cases there were 60 cases (40.5%) of real infections with the parasite. These infections distributed on different age groups, and the highest was in the reproductive age group (21-30) years and it was 40%of cases, and the parasite was detected in complicated cases such as inflammation and other symptoms (30%), followed by pregnancy(18.33%). Trichomonasis combined with elevation of vaginal pH and most of cases (31 cases out of 60 cases) detected at pH 6. Trichomonasis combined with elevation of WBCs count/ml (12000-15000 WBCs/ml) blood and pus cells in vaginal discharge، 31 pus cell/field. Using Polymerase chain reaction for amplification of 18S rRNA showed sensitivity at 100% and specificity 100% to amplify segment 312 bp. Positive results with Polymerase chain reaction appeared in samples which were negative in other tests such as direct microscopy، pH, Wiff test, Rapid test based on the immunity | ||
| Keywords | ||
| Trichomonas vaginalis; Molecular PCR; S gene | ||
|
Statistics Article View: 8 PDF Download: 4 |
||