EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES | ||
| Mesopotamia Journal of Agriculture | ||
| Article 7, Volume 40, Issue 0, March 2012, Pages 52-57 PDF (0 K) | ||
| DOI: 10.33899/magrj.2012.63474 | ||
| Author | ||
| Taha J . Al-Taha | ||
| Abstract | ||
| The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collected from ovaries obtained from Basra Slaughter House .Oocytes were matured in TCM199 (tissue culture medium 199) supplemented with10%Fetal bovin serum (FBS) to study the effect of capacitation media on their fertilizing ability. freshly ejaculated and frozen thawed spermatozoa were capacitated in 1 of 2 media .A Bracket culture medium or Earle’s balanced salt medium . Results revealed that Bracket medium with Hyalouronidase enzyme and Gentamycin instead of Penicillin antibiotics showed significant increase (P≤ 0.05) in number of in vitro fertilized and divided ewe’s oocytes as compared with the Earle’s in both fresh and frozen thawed spermatozoa . | ||
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