THE DETECTION OF ZERALENONE TOXIN IN MAIZE AND ITS DETOXIFICATION* | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 43, Issue 0, February 2013, Pages 18-26 | ||
| Authors | ||
| H. Z. Hussein; A. K. Slomy | ||
| Abstract | ||
| This study was conducted in pesticide and mycotoxin laboratory, Department of Plant Protection, College of Agriculture, University of Baghdad to detect the zeralonine mycotoxin, produced by Fusarium graminearum in maize grains. Thirteen samples of maize grains were collected from Babel, Baghdad, Waste, and Nineveh governorates, previously proved contaminated with F. graminearum were used for toxin detection. The fungal isolates obtained were grown on rice grains substrate and the zeralenone was detected by HPLC. The more zeralenone producer isolate was selected, referred to as Zer-g-Ak, and grown on rice grains for inducing zeralenone production. Maize grains were thoroughly mixed with 2g of rice grains powder contaminated with Zer-g-Ak isolate, and used for evaluating the efficiency of some detoxification agents. The efficiency of some physical (bentonite and caoaline), biological (Sacchromyces cerevisia powder) chemical (urea), magnetized water, and magnetic ring to detoxification and reduce of zeralenone were tested. Bentonite and caoaline were added to contaminated maize grains at 5% for one month, S. cerevisia powder at 0.8g/2.5ml water/500 g grains for 24 hours, maize grains were soaked in magnetized water for 24 hours, and maize grains were passed 7 times through magnetic ring. The treated grains were dried in oven at 400C, grind and used for zeralenone concentration determination by HPLC. Results showed that 4 of 13 samples were found to be contaminated with zeralenone (30.75). The passage of contaminated maize grainsthrough magnetic ring, soaked in magnetized water, treated with, fylex, bentonite, urea, S. cerevisia powder and caoaline have led to reduce zeralenone in contaminated grains by 99.4, 97.7, 92.2, 88.2, 88.2, 88.1 and 65.5% respectively. | ||
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