Effect of Lipotiechoic acid extracted from Enterococcus faecalison normal and some cancer cell lines | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 5, Issue 1, June 2012, Pages 102-107 | ||
| Authors | ||
| A. K.Mohammed; A. H. Abdalla; N. Y. Yaseen | ||
| Abstract | ||
| The current study included three parts: the first part is the isolation and identification of 12 isolates of Enterococcus faecalis from 45 different samples included 6 samples of urine, 20 samples of feces, 7 samples of skin,10 samples of Crop of the chicken and 2 samples of Liver of the sheep by using the cultural, Biochemical and serological tests from November 2010 to March 2011. Resistance of E. faecalis isolates to antibiotics were examined. Some of these isolates were resistant to 9 out of 16 antibiotics were used in this study, (100% to Streptomycin, 92% Tobramycin, 83% to Amikacin, 66% to Erythromycin, 75% Ceftazidime, 84% Augmentin, 33% Cefotaxime, 60% Tetracycline and 91.5% Nalidixic acid). On the other hand, all isolates were sensitive to Genatmicin, Chloramphenicol, Penicillin G, Ampicillin, Vancomycin, Rifampcin and Co-trimexazole. In the second part of the study, lipoteichoic acid (LTA) was extracted from E. faecalis that was isolated from feces of infected child and possessed high resistance to antibiotics by using Hot phenol, thereafter partial purification by using Sepharose CL-6B gel filtration chromatography was done. The peak of LTA was extended from fraction 8 to 18. The contents of partial purification of LTA from phosphorous 91.37 mg/ml and 0.022 mg/ml protein were measured. The third parts involved examination of cytotoxic effect of crude and partial purified LTA in normal and several cancer cell lines. Both crude and partial purified extracts at 5000 mg/ml were exhibited high inhibitory activity to Gliobl astoma (AMGM) and Ahmed-Mohammed- Nahi, 2003 (AMN-3) at exposure time 24h. reducing in the percentage of inhibition was noticed when the exposure time was increased. On the contrary, low concentration of both extracts of LTA were induced the proliferation of cancer cells of AMGM and AMN-3 at exposure time 24h. Notably, when the exposure time was increased, the proliferation of AMGM and AMN-3 was decreased. Obviously, the cytotoxicity of the both extracts) crude and partial purified) has slight effect on normal cell line, Rat Embryo fibroblast (Ref). | ||
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