ISOLATION AND IDENTIFICATION OF SOME INULINASES PRODUCING MOLDS | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 43, Issue 6, October 2013, Pages 88-96 | ||
| Authors | ||
| Israa O. G. Al-Karaquly; Aswan H. Abboud | ||
| Abstract | ||
| Sixty nine of inulinase producer molds isolates were isolated from soil and some spoiled crops, fourty seven isolates were obtained at 45°C and twenty two isolates were obtained at 30°C by cultivating them on Czapek-Dox medium with standard inulin only as a carbon source for growth surety .The isolates were screened qualitatively by using media with inulin for the best enzyme activity and quantitatively by cultivating them on media containing inulin and artichock extract to select the best isolate in inulinase production by select the isolate with high specific activity by achievement quantitatively screening in two steps ,and the isolate 49 was the highest inulinase producer at 45°C in inulinase production, in first step the activity of this isolate was 0.479u/ml and specific activity was 20.826u/mg, in second step the activity of this isolate was 0.138u/ml and specific activity was 4.451u/mg. The isolate was identified by cultivate it on special media and found that it was Aspergillus oryzae (A.oryzae) which the diameter of colony on Czapek-Dox Agar was 5-6cm at 24-26 °C with regular circular shape and yellowish green colour, it was kind of Aspergillus flavus but it is distinguish than the latest that it is non toxic and moreover it is very important in fermented foods .it is concluded that we can use solid state fermentation for inulinases production from molds instead of submerged culture for its advantages . The isolate 49 was thermophilic for growing at 45°C and it was Aspergillus oryzae (A.oryzae).We recommended to purify these enzymes and utilize them in food processing because they are thermophilic. | ||
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