Influence of nutrient medium components ,sucrose and tyrosine on callus induction , papaverine and noscapine alkaloids production on Papaver somniferum In vitro | ||
| journal of kerbala university | ||
| Article 1, Volume 8, Issue 0, January 2012, Pages 243-254 PDF (0 K) | ||
| Abstract | ||
| A study was conducted at the tissue culture lab.College of Agriculture /University of Baghdad and Drug Research Center /Health Ministry during the period Sep. 2008 till Oct.2009 . Result indicated that MS medium was superior in both fresh and dry weights of callus 155.1 ; 14.54 mg respectively , compared with B5 medium that gave 127.4 ; 11.81 mg for both fresh and dry weights of callus, respectively .Results showed that MS medium supplemented with 0.3 mg/l BA and 0.5 mg/ l of NAA gave the highest values of fresh and dry weights of callus 357.4 ; 33.71mg, respectively compared with B5 medium supplemented with the same concentration of BA and NAA which gave 253.3 ; 23.4 mg for both fresh and dry weights of callus ,respectively . Results indicated that MS medium supplemented with 30g/l of sucrose gave the highest values 548.5 ; 51.6 fresh and dry weights of callus ,respectively . However, values of both parameters were reduced with the increasing of sucrose concentration up to 60 ; 90 and120 g/L , it gave 445.5 ; 333.3 and 257.8 mg and 45.7 ; 34.1 and 24.0 mg for both fresh and dry weights of callus, respectively. MS medium supplemented with 30g/L sucrose and 20 mg/L tyrosine gave the highest values 598.5 ; 53.9 mg of both fresh and dry weights of callus, respectively . MS medium supplemented with 90g/L sucrose and 30 mg/L tyrosine gave the highest values 3.9 ; 5.9 mg/g of papaverine and noscapine for fresh callus weight, respectively . While, the lowest values of papaverine and noscapine 2.3 ; 2.5 mg/g for fresh callus weight respectively of control treatment. | ||
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