Effect of potato Solanum tuberosum and lemon Citrus sinesis extracts on callus induction and development of two local Wheat (Triticum aestivum L.) varieties | ||
| Journal of Basrah Researches (Sciences) | ||
| Article 1, Volume 37, Issue 5, October 2011, Pages 116-126 PDF (0 K) | ||
| Authors | ||
| Weam M. Hussein; Hussein K. Al-Ka; ; | ||
| Abstract | ||
| The success of plant tissue culture as a mean of plant propagation is greatly influenced by the nature of the culture medium used and the nature of its additional constituents. Adding these constituents to the nutrient medium is not cost-effective. For that reason researches focused on finding some alternative constituents that minimize cost of nutrient medium preparation without affecting its ability to enhance micropropagation processes. In this study we use plant extracts as a substitute to the commonly used additives in wheat micropropogation. We add potato and lemon extracts separately at concentrations of 3 and 6 ml/l to the nutrient medium and compare their effects on callus induction and development for two local wheat varieties named Hashimiah and Maxipac with commonly used additives as a control. We use 2, 4-D and NAA at concentrations (mg/l) 2, 1 respectively as growth regulators adding theme separately to the nutrient medium. | ||
| Keywords | ||
| Wheat; plant extracts; callus induction; growth regulator | ||
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