Isolation and Identification of the bacterium Burkholderia pseudomallei from dairy cows in the province of Al Muthanna and examination of the Sensitivity towards antibiotics | ||
| Journal of College of Education for Pure Science | ||
| Article 1, Volume 2, Issue 2, June 2012, Pages 135-148 | ||
| Author | ||
| Naer Abdulbari Alkaabi | ||
| Abstract | ||
| Isolated (26) isolation out of (82) milk samples from cows in the different regions of the province of Muthanna (Rumaythah, Suer, Mahdi, vegetables, Warka) using tubes sterile ((Scrow tube and make a variety of tests and to isolate and diagnose the bacteria B. pseudomallei were planted samples the center of the Ashdown Selective Broth, which gave the highest percentage of isolation of the bacterium B.pseudomallei (61%) and then planted on the center of Ashdown Selective Agar, where the percentage of isolation (32%) was Development and activate the colonies germ isolated from circles selectivity to save the isolates through cultivation on the center of Nutrient Agar, who gave the percentage of isolation (100%) for the purpose of developing and activating the bacteria and confirm a diagnosis of MRSA was stained cells stained Methylin blue has given proportion (87.5%), where the concentrated dye on both sides of the bacterial cell (polar cell Bipolar) as well as when it was painted stained Gram given proportion (93.7%) give pink color while were biochemical diagnostic testing for all bacterial isolates were elected on the center (ASA) test, for example the production of an enzyme Alcatalase as well as the test enzyme Al-oxidase gave the proportion of isolation (100& 84.3%) respectively on the development of germ Altoaliuand the center of the selectivity, such as central Macconky Agar and Manitol Salt Agar has given the percentage of isolation (95.1 &7%), respectively, also showed the isolates ability to liquefy gelatin by (71%) and fermented for diabetes glucose and lactose by (78 & 68%) respectively and oxidizing them by (76 & 78 %), respectively. of the bacterium the ability to solve blood type (β) and by (49%), while the remainder of the isolates (42%) are cases of blood-type (α) and (9%) were the status of the blood of type (δ) and tested the ability of bacteria to move and gave (87 % ).The results showed high sensitivity of the isolates B.pseudomallei the direction of anti-microbes of a Alkolinat represented in anti the Ciprofloxacin where the percentage of sensitivity (100%) and also for anti-Chloromphenicol was (67%) As for the anti-group macrolides represented in anti Erthromycin the isolates showed resistance to as high as resistance ratio (100%), but for a group of anti-represented in anti tetracycline Tetracycline showed the isolates were sensitive (35%). | ||
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