EXTRACTION AND PARTIAL PURIFICATION OF LIPOPOLYSACCHARIDE FROM KLEBSIELLA OXYTOCA AND STUDY OF ITS ROLE IN THE PATHOGENICITY | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 53, Issue 2, June 2012, Pages 271-278 | ||
| Authors | ||
| Raghad Q. Majeed; May T. Flayyih | ||
| Abstract | ||
| K.oxytoca bacteria was isolated from patients with urinary tract infection [UTI]from both sexes and at different ages and were identified by biochemical tests .the isolates were sensive to Amikicin Nitrofurantion and Ticarcillin Clavulinic acid and resistant to β-lactam antibiotics The isolate that gave highest percentage of resistant to antibiotics was used for LPS extraction by phenol-hot water method. LPS partial purification by gel filtration on Sepharose Cl-6B-200 showed that the percentage of carbohydrate in the partially purified LPS was 26.6% and percentage of binding protein was 0.06% and no nucleic acid was found. The LD50 of the bacteria and LPS was determined by injection of the male mice, it was 3.16X 106 cfu for bacteria and 266 μg/ml for LPS. | ||
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