BATCH AND FLOW-INJECTION SPECTROPHOTOMETRIC DETERMINATION OF SODIUM CEFOTAXIME IN PHARMACEUTICAL PREPARATIONS | ||
| Iraqi Journal of Science | ||
| Article 1, Volume 53, Issue 2, June 2012, Pages 241-249 | ||
| Authors | ||
| Mouyed Q. Al-Abachi; Hind S. Al-Ward; Yassmin H. Mohammad | ||
| Abstract | ||
| Simple and sensitive batch and Flow-injecton spectrophotometric methods (FIA) for the determination of Sodium Cefotaxime in pure form and in injections were proposed. These methods were based on a diazotization and coupling reaction between diazotized Sodium Cefotaxime and thymol in alkaline medium to form an intense red water-soluble dye that is stable and has a maximum absorption at 522 nm. A graphs of absorbance versus concentration show that Beer’s law is obeyed over the concentration range of 8-160 and 50-2500 µg.ml-1 of Sodium Cefotaxime, with detection limits of 1.56 and 1.32 µg.ml-1 of Sodium Cefotaxime for batch and FIA methods respectively. The FIA procedure sample throughput was 65 h-1. All different chemical and physical experimental parameters affecting on the development and stability of the colored product studied and the proposed methods were applied successfully on the determination of Sodium Cefotaxime injections preparations. | ||
| Keywords | ||
| Sodium Cefotaxime; Spectrophotometric determination; Thymol; Diazotization and coupling; Flow injection | ||
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