The immunomodulatory effect of Neem (Azadirachtaindica) seed aqueous, ethanolic extracts and Candida albicans cell wall mannoproteins on immune response in mice vaccinated with Brucella Rev-1 | ||
| The Iraqi Journal of Veterinary Medicine (IJVM) | ||
| Article 20, Volume 36, Issue 1, September 2012, Pages 120-127 PDF (0 K) | ||
| Authors | ||
| Tareq J. Faal; A. Hussein; ; Mohammad K Faraj; Al-Ramahy A.K | ||
| Abstract | ||
| In the present study, the immunomodulatory effect of Neem (Azadirachtaindica) seed aqueous, ethanolic extracts and Candida albicans cell wall mannoproteins on the immune response of mice vaccinated with Brucella Rev-1 vaccine was investigated. The study was conducted on two main groups(160 mice for each group), Each group was divided into eight subgroups 20 mice for each (I: treated with distilled water, II: treated with the Brucella Rev-1 vaccine, III: treated with mannoproteins, IV: treated with neem aqueous extract, V: treated with neemethanolic extract, VI, VII and VIII: treated with mannoproteins, neem aqueous extract and neemethanolic extract, respectively, then they were vaccinated with Brucella Rev-1). All these treatments were carried out on day 1 and then vaccinated with brucella Rev-1 vaccine on day 4 .Then the mice were tested as follows , on day 8 after vaccination(serum IFN-γ level), day 21 for (anti-Brucella antibody titer). The doses of both plant extracts and mannoproteins represented 10% of the calculated LD50 (neem extracts: 3.8096 g/Kg mannoproteins: 5.7144 mg/Kg), which were given subcutaneously. Mice of the second main group were injected with the immune suppressive drug prednisolone (5mg/Kg) 5 days prior to the treatments, which carried out on mice of the first main group. The results demonstrated clear immunomodulatory effects (improvement of non-specific, humoral immunity) of the tested immunomodulators in mice vaccinated with Brucella Rev-1 as compared with mice that were not treated with Neem extracts or mannoproteins. In this regard, The interferon-γ showed a significant increase (P≤ 0.01) serum level in immunomodulator-treated and -vaccinated mice in comparison with negative and positive groups, and again group VII showed the highest increase. The anti-Brucella antibodies assessed by indirect Immunoflourescent test also showed a significant increase titer in immunomodulator-treated and -vaccinated mice in comparison with negative and positive groups. Conclusion: The aqueous and ethanolicNeem seed extract reported the highest enhancement in all immunological parameters employed in comparison with mannoproteins of Candida albicans cell wall. | ||
| Keywords | ||
| Neem; Candida Albicans; Brucella Rev; Immunomodulatory | ||
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