The formation of carnation plants in Callus derived from the cultivation of cellular pods by way of landfill in the multiple droplets | ||
| Journal of Education and Science | ||
| Article 12, Volume 19, Issue 1, January 2007, Pages 76-84 PDF (0 K) | ||
| DOI: 10.33899/edusj.2007.51019 | ||
| Authors | ||
| Sahla Zidan; Jamela Rashid; Qutaba Al-Numa | ||
| Abstract | ||
| ABSTRACT Stem callus cultures of carnation (Dianthus caryophyllus) local variety were grown on asynthetic medium Murashige and Skoog (MS) medium. Optimal concentration of growth regulators were observed to be 0.5mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0.1mg/L Benzyle adenine (BA). Cell suspenion cultures worked best in liquid MS medium suspplemented with 0.5mg/L 2,4-D and 0.1mg/L BA successfully plated in agar drops is petridishes. Cell divisions led to the callus formation. Successful shoot regeneration occurred in agar solidified MS medium containing 0.06mg/L indole- acetic acid (IAA) and 1.0 mg/L Benzyle adenin (BA). Shoot were easily rooted and transferred to soil. | ||
| Keywords | ||
| carnation plants; Cultivation; multiple droplets | ||
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