Effect of Polyvinyl pyrilodine and sucrose on multiplication ratio ,elongation and number and length of roots of date palm cv. Sayer in vitro | ||
| Basrah Journal For Date Palm Research | ||
| Article 1, Volume 8, Issue 1, June 2009, Pages 1-12 PDF (0 K) | ||
| Authors | ||
| ; ; Khearallah M. A. Al-Jabary; ; | ||
| Abstract | ||
| This study was effectuated at Date Palm tissue culture laboratory. (Date Palm Research Center –Basra University) to study the Effect of Polyvinyl pyrilodine and sucrose on multiplication ratio ,elongation and number and length of roots of date palm cv. Sayer in vitro .different concentration of PVP was tested (0,1,2,3,4 g/L ) and different concentration of sucrose(30,40,50,60,70)G/LThese explants were cultured on nutrient medium supplemented 3 mg/l NAA and cytokines (2iP) 10 mg/l. Cultures were incubated in lights at 1000 lux at periods16 hours daily at 27±1 c0 and sub cultured was done every four weeks the results showed that: 1- Results showed that the use 2 g/l (PVP) was increased of multiplication ratio was reached(8.6%) and elongation(6.3cm) and led to have higher number of roots was reached (3.6) root/plantlet compared to the other concentration. While use 1 g/l led to have higher length of roots was reached 4.3 cm. 2-Used of 60 g/l sucrose was increased the multiplication ratio was reached (5.6%). and elongation (6.8cm)and led to have higher number of roots was reached (3.6) root/plantlet . while use 70 g/l led to have higher length of roots was reached 5.8 cm. significant compared to the other concentration 3-Results showed that the use(1 g /l PVP and 60 g/l sucrose ) was increased the multiplication ratio which (8.2%) and elongation (7.1cm)and led to have higher number of roots which (4.3) root/plantlet .significantly compared to the other treatments. While the treatment (2 g /l PVP and 60 g/l sucrose) superior significantly in the average of root length which 5.9 cm compared of other treatments . | ||
| Keywords | ||
| Polyvinyl pyrilodine; Multiplication; date palm | ||
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