A. M. Alanssari, S., S Al-Naaimi, A., K. Al-Janabi, M., M. Saleh, E., Salmo, N., M.J.Al-Khateeb, S., H. Nasser, T., Abdul Razzaq Mesheimish, B., Mohammad Batarfi, A., A. Al-Haidary, B., Sh. Al-Obeidy, E., Sataaa, J., M. Saleh, E., Kh. Al-Adhami, N., Mohammed Batarfi, A., E. Hassen, A., T. George, N. (2007). In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients. Alustath, 49(4), 394-399.
Saeeda A. M. Alanssari; Ahmed S Al-Naaimi; Muhi K. Al-Janabi; Eman M. Saleh; Nabil Salmo; Shatha M.J.Al-Khateeb; Tharwet H. Nasser; Bahir Abdul Razzaq Mesheimish; Ali Mohammad Batarfi; Batool A. Al-Haidary; Eman Sh. Al-Obeidy; Jaafer Sataaa; Eman M. Saleh; Nadhia Kh. Al-Adhami; Ali Mohammed Batarfi; Affan E. Hassen; Nameer T. George. "In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients". Alustath, 49, 4, 2007, 394-399.
A. M. Alanssari, S., S Al-Naaimi, A., K. Al-Janabi, M., M. Saleh, E., Salmo, N., M.J.Al-Khateeb, S., H. Nasser, T., Abdul Razzaq Mesheimish, B., Mohammad Batarfi, A., A. Al-Haidary, B., Sh. Al-Obeidy, E., Sataaa, J., M. Saleh, E., Kh. Al-Adhami, N., Mohammed Batarfi, A., E. Hassen, A., T. George, N. (2007). 'In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients', Alustath, 49(4), pp. 394-399.
A. M. Alanssari, S., S Al-Naaimi, A., K. Al-Janabi, M., M. Saleh, E., Salmo, N., M.J.Al-Khateeb, S., H. Nasser, T., Abdul Razzaq Mesheimish, B., Mohammad Batarfi, A., A. Al-Haidary, B., Sh. Al-Obeidy, E., Sataaa, J., M. Saleh, E., Kh. Al-Adhami, N., Mohammed Batarfi, A., E. Hassen, A., T. George, N. In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients. Alustath, 2007; 49(4): 394-399.

In Vitro Fertilization of Immature oocytes by Testicular Sperm: Animal Model for Azoospermic Infertile Patients

Journal of the Faculty of Medicine
Article 1, Volume 49, Issue 4, December 2007, Pages 394-399
Authors
Saeeda A. M. Alanssari; Ahmed S Al-Naaimi; Muhi K. Al-Janabi; Eman M. Saleh; Nabil Salmo; Shatha M.J.Al-Khateeb; Tharwet H. Nasser; Bahir Abdul Razzaq Mesheimish; Ali Mohammad Batarfi; Batool A. Al-Haidary; Eman Sh. Al-Obeidy; Jaafer Sataaa; Eman M. Saleh; Nadhia Kh. Al-Adhami; Ali Mohammed Batarfi; Affan E. Hassen; Nameer T. George
Abstract
Background: Men with azoospermia require testicular and epididymal sperm
aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate
an increase number of immature oocytes after induction of ovulation.
The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of
the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be
of therapeutic value for the treatment of azoospermic patients.
Objectives: The goal of the study was to develop an animal model for the treatment of
infertile couples with obstructive azoospermia and immature oocytes.
Materials and Methods: Canine ovaries and testes were collected from a local animal
clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue
culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS.
The normality of the fertilized oocytes was studied.
Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved
from the vas deference compared to ES and TS. The concentration of the sperm was
significantly higher (P<0.05) in the testes compared to epididymis and vas deference while
VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte
percentage was significantly higher (P

Keywords

IVM
---
IVF
---
Testicular Sperm
---
Animal model
---
Azoospermia
Keywords
IVM; IVF; Testicular Sperm; Animal model; azoospermia; Poisoning; childhood; inpatients; drugs; organic solvents; Baghdad; DM patients; HLA; GAD; Angiogenesis; colorectal tumors; anti CD; Bladder Cancer BC; Human Leukocyte Antigen HLA; BNP; Dysfunction; Heart failure; Antibiotic use; Surgerical operation; Hadhramout; Anti; CVB; Polio IgG; Adeno IgG; head injury; Trace Elements; Glasgow Coma Scale; Oxidative stress; Exercise; Lipid profile; Anatomical study; Hippocampus; blood; supply
Statistics
Article View: 112
PDF Download: 6


Journal Management System. Powered by ejournalplus.com