The Effect of Increasing Concentration of Copper and Myo-Inisitol in In Vitro Morphogenic Response of Grape (Vitis vinifera L.) | ||
| Rafidain Journal of Science | ||
| Article 4, Volume 23, Issue 4, June 2012, Pages 36-43 PDF (0 K) | ||
| DOI: 10.33899/rjs.2012.44409 | ||
| Author | ||
| Gharbia H. Daniel | ||
| Abstract | ||
| It was found that the increased levels of copper and myo-inisitol in the culture medium was positively effective in increasing the morphogenic response of grape plants Kamali cultivar till the level of 0.025+ 200 mg/l from both compounds respectively. Since the use of this combined level caused to achieve the highest number of shoots (10 shoots/ explant) and leaves (25.75 leaves/ explant) at shoot multiplication stage as well as achieving the highest number of roots (3.08 roots/ explant) at root formation stage. The use of these levels of copper and myo-inisitol did not show any aberrant morphology appearance but the use of higher elevated levels till three times, gradually decreased the morphological response by giving lower values of the numbers of shoots, leaves and roots. At acclimatization stage, the produced plants were successfully transferred to the out-air conditions with a high survival rate reached to 85%. | ||
| Keywords | ||
| Grape; copper; myo; inisitol; tissue cultures | ||
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