Spectrophotometric Determination of Tetracycline by Coupling with Diazotised 4-Aminoantipyrine in Presence of Cetylpyridinium Chloride | ||
| Rafidain Journal of Science | ||
| Article 7, Volume 23, Issue 3, April 2012, Pages 72-84 PDF (0 K) | ||
| DOI: 10.33899/rjs.2012.44397 | ||
| Authors | ||
| Rasha J. Al-Ashow; Nabeel S. Othman | ||
| Abstract | ||
| A spectrophotometric method for the determination of microgram amounts of tetracycline -HCl(TCH) has been proposed. The method is based on coupling of tetracycline-HCl with diazotized 4-aminoantipyrine in presence of cetylpyridinium chloride in alkaline medium. The molar absorptivity of the formed dye reached to 1.92×104 l.mol-1. cm-1 at 537 nm and Beer's law is obeyed within the range of 10-300 μg of tetracycline-HCl /10 ml (i.e.1.0 – 30 ppm). The colour reaction was highly stable, a relative error ranged from - 2.5 to + 0.21 % and a relative standard deviation of ± 0.37 to ± 0.73 %, depending on concentration level of tetracycline-HCl. The proposed method has been applied successfully to the determination of tetracycline-HCl in pharmaceutical preparations. | ||
| Keywords | ||
| Tetracycline; Hcl; aminoantipyrine; diazo; Coupling; spectrophotometry | ||
|
Statistics Article View: 334 PDF Download: 696 |
||