G. Sweedan, E. (2012). Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria. Alustath, 4(1), 15-18. doi: 10.37652/juaps.2010.43881
Enass G. Sweedan. "Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria". Alustath, 4, 1, 2012, 15-18. doi: 10.37652/juaps.2010.43881
G. Sweedan, E. (2012). 'Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria', Alustath, 4(1), pp. 15-18. doi: 10.37652/juaps.2010.43881
G. Sweedan, E. Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria. Alustath, 2012; 4(1): 15-18. doi: 10.37652/juaps.2010.43881

Study the effect of Antibiotics on pyocyanin production from Pseudomonas aeruginosa and pyocyanin as Antibiotic against different pathogenic bacteria

Journal of University of Anbar for Pure Science
Article 6, Volume 4, Issue 1, April 2010, Pages 15-18    PDF (271.11 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2010.43881
Author
Enass G. Sweedan*
University of Baghdad - college of science.
Abstract
aeruginosa was cultured on nutrient agar containing different antibiotics,the growth and pigment of
bacterial colonies were treated. Azithromycin killed the bacteria on low concentration,colonies of P. aeruginosa
treated with Ampicillin,Amoxycillin,and Cefixime appeared light green .Cephalexin and Azithromycin led to
formation of light green-yellow colonies,while Doxycillin ,and Clindamycin appeared green ,but there was slight
change in colonies color.
The pigment(pyocyanin) was extracted from the untreated cells of bacteria and its effect as antibiotic used against
many different pathogenic bacteria have been studied,the pigment inhibited E. coli, Acinetobacter,Staphylococcus
auraus ,and Streptococcus pneumoniae but not Klebsiella pneumoniae ,and Proteus vulgaris
Keywords
Pseudomonas aeruginosa; Pyocyanin; Antibiotics
References
  1. Hasset, J. D.; Charniga, L., Bean, K.; Ohman, D. E.; and cohen, M.S. (1992). Response of Pseudomonas aeruginosa to Pyocyanin: Mechanisms of Resistance, Antioxodant Defenses, and Demonstration of a Manganese-Cofacted Superoxide Dismutase.J of Infecect. and Immunity. 60(2) :328-336.
  2. Mavrodi , D. V. and Thomashow ,L. S. (2001) .Functional analysis of Genes of Biosynthesis of Pyocyanin and phenazine-1-carboximide from Pseudomonas aeruginosa PA01.J. of Bacteriolog. 21:6454-6465.
  3. Young, G.(1947).Pigment production and antibiotic activity in cultures of Pseudomonas aeruginosa .J. Bactacteriology.54:109-117.
  4. Hassan,H.M. and Fridovich,I. (1980).Mechanism of antibiotic action of Pyocynin.J. of Bacteriology. 141:156-163.
  5. Prithiviraj, B.; Bais, H.; Weir, T.; Suresh, B.; Najarro, E.; Dayakar, B.; Schweizer, J.; Vavanco, J. (2005).Down regulation of virulence factors of Pseudomonas aeruginosa by salicylic acid attenuates its virulence on Arabidopsis thaliana and Caenorhabditis elegans. Infect. And Immun. 73(9):5319-5328.
  6. Vinnckx, T.; Wei, Q.; Matthijs, S.A. and Crnelis,P. (2010). The Pseudumonas aeruginosa oxidative stress regulator OxyR influence production of pyocyanin and rhamnolipids: productive role of pyocyanin.J. of Microbiology. 156:678-686.(abstract)
  7. Garrity, M.G.; Bell, J.A. and Liburn, T.(2005). Pseudomonales In: Bergy´s manual of systematic bacteriology. (Brenner, D.J.; Krieg, N. R. and Staley, J. T. (editors)).323-352. 2nd edition .Springer.
  8. Essar,D.W.;Eberly,L.;Hardero,A. and Crawford. (1990). Identification and characterization of genes for a second nanthranlate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranlate synthetase and evolutionary implications. J. of Bacteriology.172:884-900.
  9. Molinari,G.;Guzmán, C.A. ; Pesce, A. and Schito, C.(1993). Inhibition of Pseudomonas aeruginosa virulence factors sub inhibitory concentrations of azithromycin and other macrolide antibiotics J.of Antimicrob. Chemother. 31:681-688.(abstract)
10. Shen,.; Shi, Y.; Zhang, D.; Wei, J.; Surette, M.G.; and Duan, K. (2008). Modulation of secreted virulence factors genes by sub inhibitory concentrations of antibiotics in Pseudomonas aeruginosa .J. of Microbiology.46(4):441-447. (abstract)

11. Kong,K.;J ayawardena ,S.R.; Indulkar, S.D.; Puerto, A.; Koh, C.; Hoiby, N.and Mathee, K.(2005). Pseudomonas aeruginosa Amp R Is a Global Transcriptional factor That Regulates Expression of Amp C and Pox β-Lactamases ,Protases, Quoraum sensing and other Virulence Factors.       J. ntimicrobial. Agents and chemother. 49(11):4567-4575.

12. Portoles, A.; Espinosa, M.; and Hidalgo, A.(1971). Pencillin and Polymyxin effects on the chromogensis of Pseudomonas aeruginosa strains of antibiotics.XXIV(4).266-269.

13. Hassett, D, J.; Britigan, T.; Svendesen, T.; Rosen, G.M.; and Chen, M.S. (1987). Bacteria from intracellular free radicals in response to parquet and streptonigrin: demonstration of the potency of hydroxyl radical. J. of Biol.Chem.262:13404-13408.

14. Baron, S.S. ;Terranova, G. and Rowe, J.J. (1989). Molecular Mechanism of the Antimicrobial Action of Pyocanin .Current Microbiology.18:223-240. (abstract).

Statistics
Article View: 271
PDF Download: 130


Journal Management System. Powered by ejournalplus.com