Effect of NaCl on Callus growth of Catharanthus roseus and its vincristine and vinblastine alkaloids content. | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 4, Issue 1, June 2012, Pages 88-92 | ||
| Abstract | ||
| An experiment was conducted at Plant Tissue Culture Laboratory / Horticulture Department / Agriculture College / Kufa University during 2008-2009 to study the effects of NaCl at concentrations ( 0, 25 , 50 or 75 mM ) concentrations on Catharanthus roseus cv. rosea callus growth initiated from in vitro multiple shoots, and its total indole alkaloids, vincristine and vinblastine contents. Results showed significant differences in content of callus in total indole alkaloids, vincristine and vinblastine cultured at different NaCl salt concentrations in MS medium. NaCl treatments gave a significant increase in these parameters as compared with the control ( with out NaCl ). The highest amount of total indole alkaloids, vincristine and vinblastine (3.18 mg / gm callus dry weight, 5.12 µg / gm callus dry weight and 6.11 µg / gm callus dry weight ) respectively was found in the callus cultured in MS medium that supplied with 50 mM NaCl as compared with the lowest values(1.11 mg/ gm callus dry weight , 1.56 µg/ gm callus dry weight and 3.76 µg / gm callus dry weight ) in the control. | ||
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